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Using Chemistry to Investigate the Molecular Consequences of Protein Ubiquitylation

Authors

  • Tharindumala Abeywardana,

    1. Department of Chemistry, University of Southern California, 840 Downey Way, Los Angeles, CA 90089 (USA)
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  • Prof. Dr. Matthew R. Pratt

    Corresponding author
    1. Department of Chemistry, University of Southern California, 840 Downey Way, Los Angeles, CA 90089 (USA)
    2. Department of Molecular and Computational Biology, University of Southern California, 840 Downey Way, Los Angeles, CA 90089 (USA)
    • Department of Chemistry, University of Southern California, 840 Downey Way, Los Angeles, CA 90089 (USA)===

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Abstract

Chemistry has long played an indispensable role in biological discovery through the synthesis of homogeneous, structurally defined material. With continuing advances in the area of synthetic protein chemistry, chemists are able to prepare increasingly large and complex proteins that have enabled key biochemical experiments. Here, we describe some of the chemical methods that have been applied to the synthesis of ubiquitylated proteins, as ubiquitylation is a crucial post-translational modification that mediates a variety of important biological effects on substrate proteins.

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