Photocontrol of Protein Activity in Cultured Cells and Zebrafish with One- and Two-Photon Illumination (pages 653–663)
Deepak Kumar Sinha, Pierre Neveu, Nathalie Gagey, Isabelle Aujard, Chouaha Benbrahim-Bouzidi, Thomas Le Saux, Christine Rampon, Carole Gauron, Bernard Goetz, Sylvie Dubruille, Marc Baaden, Michel Volovitch, David Bensimon, Sophie Vriz and Ludovic Jullien
Version of Record online: 24 FEB 2010 | DOI: 10.1002/cbic.201000008
The inner light: We have implemented a photochemical method to control the activity of proteins linked to the ERT2 receptor in live zebrafish embryos. Using fluorescent proteins inactivated by association with endogenous chaperone complex (CC), we showed that photoreleasing the cyclofen inducer led to fast protein photoactivation. Colocalization experiments suggest that light-induced activation can be restricted to a few targeted cells.