Subcellular Protein Localization by Using a Genetically Encoded Fluorescent Amino Acid (pages 1818–1821)
Dr. Godefroid Charbon, Dr. Eric Brustad, Kevin A. Scott, Prof. Dr. Jiangyun Wang, Prof. Dr. Anders Løbner-Olesen, Prof. Dr. Peter G. Schultz, Prof. Dr. Christine Jacobs-Wagner and Prof. Dr. Eli Chapman
Article first published online: 16 JUN 2011 | DOI: 10.1002/cbic.201100282
CouAA-lescence: A fluorescent amino acid (CouAA) was site-specifically incorporated into FtsZ, the bacterial tubulin homologue, in living cells. FtsZCouAA is seen at the cleavage furrow during cell division to form the Z-ring. These studies provide the first example of a fully functional protein to be visualized in vivo by using a genetically incorporated fluorescent amino acid.