A Triple Spin-Labeling Strategy Coupled with DEER Analysis to Detect DNA Modifications and Enzymatic Repair (pages 2560–2563)
Dr. Mélanie Flaender, Dr. Giuseppe Sicoli, Dr. Samia Aci-Seche, Thomas Reignier, Dr. Vincent Maurel, Christine Saint-Pierre, Dr. Yves Boulard, Dr. Serge Gambarelli and Dr. Didier Gasparutto
Article first published online: 7 OCT 2011 | DOI: 10.1002/cbic.201100550
In a spin: Spin-labeled oligonucleotides produced by click chemistry can be studied by EPR, by using a DEER sequence. This was used to test a complex triple-labeling strategy with damaged DNA. Extensive and accurate analysis of DNA structure and enzymatic repair processes were performed after digestion by EndoIV (see scheme). Modified DNA structures and DNA–protein interactions can now be readily studied.