Activity-Based Profiling of Retaining β-Glucosidases: A Comparative Study (pages 1263–1269)
Dr. Martin D. Witte, Marthe T. C. Walvoort, Kah-Yee Li, Wouter W. Kallemeijn, Wilma E. Donker-Koopman, Dr. Rolf G. Boot, Prof. Dr. Johannes M. F. G. Aerts, Dr. Jeroen D. C. Codée, Prof. Dr. Gijsbert A. van der Marel and Prof. Dr. Herman S. Overkleeft
Article first published online: 28 APR 2011 | DOI: 10.1002/cbic.201000773
Compare and contrast: 2-Deoxy-2-fluoro glucosides and cyclitol derivatives have been tested for their ability to irreversibly bind to retaining β-glucosidases. One- and two-step labeling protocols are compared, and the superiority of cyclophellitol probes in activity-based protein profiling is established.