Thiogalactopyranosides are Resistant to Hydrolysis by α-Galactosidases (pages 1673–1679)
Dr. Dietlind Adlercreutz, Dr. Yayoi Yoshimura, Dr. Karin Mannerstedt, Dr. Warren W. Wakarchuk, Dr. Eric P. Bennett, Prof. Norman J. Dovichi, Prof. Ole Hindsgaul and Prof. Monica M. Palcic
Version of Record online: 27 JUN 2012 | DOI: 10.1002/cbic.201200155
Keep it sweet: Fluorescent glycosides containing either galactose or 5-thiogalactose as the terminal sugar were enzymatically synthesized, and hydrolysis was measured by capillary electrophoresis. The hydrolytic resistance of 5-thiogalactose analogues to human α-galactosidase A was demonstrated both in vitro and in vivo, suggesting that 5-thiogalactosides may be useful tools for the study of anabolic pathways in cell extracts or in single cells.