A Versatile and Colorful Screening Tool for the Identification of Arabinofuranose-Acting Enzymes (pages 1885–1888)
Dr. Vinciane Borsenberger, Dr. Fernando Ferreira, Dr. Annick Pollet, Dr. Emmie Dornez, Dr. Marie-Laure Desrousseaux, Dr. Stéphane Massou, Prof. Dr. Christophe M. Courtin, Dr. Michael J. O'Donohue and Dr. Régis Fauré
Article first published online: 6 AUG 2012 | DOI: 10.1002/cbic.201200394
Selecting wall-nibblers: Three 4-nitrocatechol derivatives were designed to facilitate high-throughput screening of arabinofuranose hydrolases, enzymes that typically digest plant cell walls. The designed compounds can be used in solid and liquid media, and, importantly, one allows the specific detection of AXH-d, a specialized enzyme that only releases L-arabinose from disubstituted D-xylosyl moieties.