Papain-Specific Activating Esters in Aqueous Dipeptide Synthesis (pages 1319–1326)
Roseri J. A. C. de Beer, Barbara Zarzycka, Michiel Mariman, Helene I. V. Amatdjais-Groenen, Marc J. Mulders, Dr. Peter J. L. M. Quaedflieg, Dr. Floris L. van Delft, Dr. Sander B. Nabuurs and Prof. Floris P. J. T. Rutjes
Article first published online: 21 MAY 2012 | DOI: 10.1002/cbic.201200017
A set of new activating esters was evaluated in an enzymatic, papain-catalyzed approach to synthesizing dipeptides under aqueous conditions. In particular, benzyl and dimethylaminophenyl esters yielded the corresponding dipeptides in several instances in high yields and with relatively small levels of hydrolysis. These results were also interpreted by computational docking analysis.