Platinum(II)-Based Coordination Compounds as Nucleic Acid Labeling Reagents: Synthesis, Reactivity, and Applications in Hybridization Assays (pages 573–583)
R. J. Heetebrij, E. G. Talman, M. A. v. Velzen, R. P. M. v. Gijlswijk, S. S. Snoeijers, M. Schalk, J. Wiegant, F. v.d. Rijke, R. M. Kerkhoven, A. K. Raap, H. J. Tanke, J. Reedijk and H.-J. Houthoff
Article first published online: 27 JUN 2003 | DOI: 10.1002/cbic.200200498
Nucleic acid labeling reagents: The synthesis of a series of platinum complexes (see scheme) modified with fluorescent and hapten tags is described. The reactivity profile and thus the mode of binding towards nucleic acids were studied. The tethered reporter group plays a significant role in reactivity towards mono- and polynucleotides. The application of these complexes in nucleic acid labeling and hybridization assays is illustrated in both a fluorescent in situ hybridization (FISH) and on a cDNA microarray.