Generation of Novel Landomycins M and O through Targeted Gene Disruption (pages 675–678)
Andriy Luzhetskyy, Lili Zhu, Miranda Gibson, Marta Fedoryshyn, Clemens Dürr, Carsten Hofmann, Dirk Hoffmeister, Bohdan Ostash, Cynthia Mattingly, Val Adams, Victor Fedorenko, Jürgen Rohr and Andreas Bechthold
Version of Record online: 6 APR 2005 | DOI: 10.1002/cbic.200400316
New deoxylandomycins. Targeted inactivation of the landomycin reductase gene, lanZ4, and the oxygenase gene, lanZ5, in Streptomyces cyanogenus S136 resulted in the formation of new derivatives that lack the unique hydroxyl group in the C-11 position. The new landomycins posses much weaker antitumor activities; this highlights the importance of the C-11 hydroxyl group of the angucycline moiety. Our data suggest that that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis, as shown in the scheme.