Anti-Carbohydrate Antibodies Elicited by Polyvalent Display on a Viral Scaffold (pages 1455–1462)
Eiton Kaltgrad, Sayam Sen Gupta, Sreenivas Punna, Cheng-Yuan Huang, Aileen Chang, Chi-Huey Wong, M. G. Finn and Ola Blixt
Version of Record online: 6 AUG 2007 | DOI: 10.1002/cbic.200700225
Presentation is important. Many diseases are marked by the production of unusual cell-surface glycans, but carbohydrates often evade the immune system. The display of oligosaccharides on the exterior protein surface of a virus particle (see image) elicits strong immune responses in chickens to give large quantities of selective anti-glycan polyclonal antibodies. Binding specificities were determined by using glycan arrays, which provide comprehensive comparisons between glycan-binding proteins.