Site-Specific Functionalization of Proteins by Organopalladium Reactions (pages 232–238)
Koichiro Kodama, Seketsu Fukuzawa, Hiroshi Nakayama, Kensaku Sakamoto, Takanori Kigawa, Takashi Yabuki, Natsuko Matsuda, Mikako Shirouzu, Koji Takio, Shigeyuki Yokoyama and Kazuo Tachibana
Version of Record online: 29 DEC 2006 | DOI: 10.1002/cbic.200600432
Accommodating the organopalladium conditions. An aryl iodide tag on the Ras protein was chemoselectively modified by Mizoroki–Heck and Sonogashira reactions (see figure). The Ras protein retained its biological activity under organopalladium conditions. These results were confirmed by Western blot, LC-MS, and in vitro pull-down analyses.