Elaboration of Neosamine Rings in the Biosynthesis of Neomycin and Butirosin (pages 283–288)
Fanglu Huang, Dieter Spiteller, Neil A. Koorbanally, Yanyan Li, Nicholas M. Llewellyn and Jonathan B. Spencer
Article first published online: 8 JAN 2007 | DOI: 10.1002/cbic.200600371
The conversion of paromamine to neamine in the neomycin biosynthetic pathway has been shown to be catalyzed by joint activity of Neo-11, a glucosaminyl-6′-oxidase, and Neo-18, a 6′-oxoglucosaminyl:L-glutamate aminotransferase. Similar results were obtained by replacing Neo-18 with BtrB, a homologue of Neo-18 encoded in a butirosin biosynthetic gene cluster. Additionally, Neo-18 has been shown to be a dual-function aminotransferase responsible for the addition of both the 6′- and 6′′′-amino groups in neomycin (1).