Converting Cytochrome c into a Peroxidase-Like Metalloenzyme by Molecular Design (pages 607–609)
Zhong-Hua Wang, Ying-Wu Lin, Federico I. Rosell, Feng-Yun Ni, Hao-Jie Lu, Peng-Yuan Yang, Xiang-Shi Tan, Xiao-Yuan Li, Zhong-Xian Huang and A. Grant Mauk
Article first published online: 27 FEB 2007 | DOI: 10.1002/cbic.200600547
Even better than the real thing. Based on molecular design, an electron-transfer hemoprotein, cytochrome c, was converted into a peroxidase-like enzyme by introduction of a distal histidine into the heme pocket. The cytochrome c variants obtained, Tyr67His and Tyr67His/Met80Val (see figure), showed much higher peroxidase activities than wild-type cytochrome protein. More interestingly, the kcat/Km values of the new hemoproteins were higher than that of wild-type horseradish peroxidase.