Synthesis, Conformation, and Activity of Human Insulin-Like Peptide 5 (INSL5) (pages 1816–1822)
Mohammed Akhter Hossain, Ross A. D. Bathgate, Chze K. Kong, Fazel Shabanpoor, Suode Zhang, Linda M. Haugaard-Jönsson, K. Johan Rosengren, Geoffrey W. Tregear and John D. Wade
Article first published online: 24 JUN 2008 | DOI: 10.1002/cbic.200800113
Ready for a shot: The A and B chains of human insulin-like peptide 5 (INSL5) are “difficult sequences”, however, we managed to successfully prepare these by SPPS after protocol modification. Use of regioselective cysteine thiol protecting groups allowed subsequent sequential disulfide bond formation and chain combination. The resulting synthetic INSL5 was obtained in good overall yield and will be a valuable tool in determining its biological function.