Self-Cleavable Bioluminogenic Luciferin Phosphates as Alkaline Phosphatase Reporters (pages 714–718)
Wenhui Zhou, Christine Andrews, Jianquan Liu, John W. Shultz, Michael P. Valley, Jim J. Cali, Erika M. Hawkins, Dieter H. Klaubert, Robert F. Bulleit and Keith V. Wood
Article first published online: 5 FEB 2008 | DOI: 10.1002/cbic.200700644
After glow. Reactive chemical adaptors were used to stabilize bioluminogenic phosphates for monitoring alkaline phosphatase (AP) activity (see scheme). The 1,6-elimination based luciferin phosphate exhibited ultrasensitive ability to detect ∼10−22 mol of AP enzyme in a homogeneous solution, and picograms of protein in an immunoassay.