Synthesis and Application of Fluorescein- and Biotin-Labeled Molecular Probes for the Chemokine Receptor CXCR4 (pages 1154–1158)
Shinya Oishi, Ryo Masuda, Barry Evans, Satoshi Ueda, Yukiko Goto, Hiroaki Ohno, Akira Hirasawa, Gozoh Tsujimoto, Zixuan Wang, Stephen C. Peiper, Takeshi Naito, Eiichi Kodama, Masao Matsuoka and Nobutaka Fujii
Version of Record online: 15 APR 2008 | DOI: 10.1002/cbic.200700761
Accurate labels: Molecular probes for chemokine receptor CXCR4 were derived from polyphemusin II CXCR4 antagonists (see figure: X=fluorescein, Alexa Fluor 488 or biotin–6-aminocaproic acid). Flow cytometry and confocal microscopy (see image of cells stained with a fluorescence-labeled peptide) demonstrated the selective binding of these probes to the CXCR4 receptor, but not to CXCR7.