In vivo Mutational Analysis of the Mupirocin Gene Cluster Reveals Labile Points in the Biosynthetic Pathway: the “Leaky Hosepipe” Mechanism (pages 1500–1508)
Ji'en Wu, Joanne Hothersall, Carlo Mazzetti, Yvonne O'Connell, Jennifer A. Shields, Ayesha S. Rahman, Russell J. Cox, John Crosby, Thomas J. Simpson, Christopher M. Thomas and Christine L. Willis
Version of Record online: 8 MAY 2008 | DOI: 10.1002/cbic.200800085
Metabolic leaks: Blocks in the modular polyketide synthase assembly line induced by mutation of components of the HMG CoA analogue (mupH) containing gene cassette and other tailoring genes result in a common phenotype in which both mupirocin H and the novel metabolite mupiric acid are produced. This has important implications for understanding the dynamics of intermediate flow and interpretation of mutational analysis experiments in the generic AT-less polyketide family of metabolites.