Directed differentiation of human embryonic stem cell-line HUES9 to dopaminergic neurons in a serum-free defined culture niche
Article first published online: 19 NOV 2012
© 2012 International Federation for Cell Biology
Cell Biology International
Volume 37, Issue 1, pages 54–64, January 2013
How to Cite
Datta, I., Ganapathy, K., Tattikota, S. M. and Bhonde, R. (2013), Directed differentiation of human embryonic stem cell-line HUES9 to dopaminergic neurons in a serum-free defined culture niche. Cell Biology International, 37: 54–64. doi: 10.1002/cbin.10012
- Issue published online: 20 DEC 2012
- Article first published online: 19 NOV 2012
- Accepted manuscript online: 9 NOV 2012 07:13AM EST
- Manuscript Accepted: 17 SEP 2012
- Manuscript Received: 4 JUL 2012
Additional supporting information may be found in the online version of this article.
|cbin10012-sup-0001-Table.doc||117K||Supporting Table 1. List of primer sequences used for RT-PCR.|
|cbin10012-sup-0002-Figure.doc||695K||Supporting Figure 1. (a) Represents the bright field image of HUES9 colony at day 4. (b-f) Representative immunofluorescence images of HUES9 stained positive (FITC; green) for pluripotent markers Oct4, Nanog, Sox2, SSEA4 and TRA-1-60. Cell nuclei were counterstained with DAPI (blue); magnification 10X10.|
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