Engraftment of genetically modified human amniotic fluid-derived progenitor cells to produce coagulation factor IX after in utero transplantation in mice
Article first published online: 15 MAR 2013
© 2013 International Federation for Cell Biology
Cell Biology International
Volume 37, Issue 5, pages 420–429, May 2013
How to Cite
Yang, C.-M., Gong, X.-L., Qiu, J., Tang, H.-X., Gong, Z.-J., Huang, S.-Z. and Zeng, F. (2013), Engraftment of genetically modified human amniotic fluid-derived progenitor cells to produce coagulation factor IX after in utero transplantation in mice. Cell Biology International, 37: 420–429. doi: 10.1002/cbin.10037
- Issue published online: 15 APR 2013
- Article first published online: 15 MAR 2013
- Accepted manuscript online: 4 JAN 2013 04:39AM EST
- Manuscript Accepted: 23 DEC 2012
- Manuscript Received: 30 NOV 2012
- National Natural Science Foundation of China. Grant Number: 81125003
- Hi-Tech Research and Development Program of China. Grant Number: 2011AA020116
- China National Basic Research Program. Grant Number: 2010CB945200
- Science and Technology Committee of Shanghai Municipality. Grant Numbers: 12XD1406500, 10140900200
Additional supporting information can be found in the online version of this article:
Figure S1. Morphology of amniotic fluid-derived progenitor cells. (A) The appearance and growth of MSC-like cell colonies on the fifth day of culturing. (B) The fibroblast-like AFPC colonies grew to confluence in the first passage culture. (C) AFPC at passage 3 appeared homogeneous. (D) AFPC at passage 12 remained fibroblast-like.
Figure S2. AFPCs from all three patients expressed NANOG, Oct4 and SOX2 mRNA. RT-PCR analyses detected NANOG, Oct4, and SOX2 mRNA in cultured AFPCs. hESCs were used as a positive control, and 293 T cells were used for negative control. β-Actin served as the normalization reference.
Figure S3. Immunocytochemical analyses revealed that most AFPCs expressed the intracellular embryonic marker proteins SOX2 and NANOG, but lacked signal for OCT4.
Figure S4. Immunocytochemical analyses revealed that most AFPCs expressed the embryonic surface markersSSEA4 and TRA-1-60, but notTRA-1-81.
Figure S5. Adipogenesis and osteogenesis of amniotic fluid progenitor cells (magnification 200×). (A) hAFPCs were cultured in adipogenic medium for 3 weeks. (B) Adipogenic differentiation detected by Oil Red O staining of lipid-rich cytoplasmic vacuoles (red). (C) hAFPCs were cultured in osteogenic medium for 3 weeks. (D) Osteogenic differentiation detected by staining for alkaline phosphatase enzyme activity (blue).
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