SEARCH

SEARCH BY CITATION

Additional supporting information may be found in the online version of this article.

FilenameFormatSizeDescription
cbin10043-0001-sm-SupFig-S1.tif99KFigure S1. Expression and affinity purification of fusion proteins GST-heRF3b(lane 1 and 2), His-hSVV(lane 3 and 4), His-heRF3a-F3 (lane 5 and 6), and GST-hSVV (lane 7 and 8), and SDS–PAGE (10%) analysis of the fusion protein. The molecular weights of the proteins are ∼94.9, 17.2, 56.6 and 42.4 kDa, respectively.
cbin10043-0002-sm-SupFig-S2.tif597KFigure S2. N-terminal domain (NTD) sequence divergence of GSPT1 (eRF3a) and GSPT2 (eRF3b). GSPT1-1 and GSPT1-3 represent two isotypes of GSPT1. The dark-grey highlighted region represents the possible IAP-binding motif (Hegde et al., 2003); the arrow indicates the truncation site used to construct the mutant eRF3s. The yellow frame indicates the random coil secondary structure, whereas the red frame regions indicate α-helix secondary structure.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.