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Atomic force microscopy of chronic lymphatic leukaemia cells activation induced by Staphylococcus aureus

Authors

  • Shisong Dong,

    1. Department of Chemistry, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China
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  • Qiulan Wang,

    1. Department of Chemistry, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China
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  • Shaoyang Sun,

    1. Department of General Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
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  • Yuanwei Liang,

    1. Department of Chemistry, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China
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  • Jinhuan Jiang,

    1. Department of Chemistry, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China
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  • Li Liu,

    1. Department of Chemistry, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China
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  • Anbin Hu,

    Corresponding author
    • Department of General Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
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  • Jiye Cai

    Corresponding author
    • Department of Chemistry, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China
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Corresponding authors: e-mail: anbinhu@163.com (Anbin Hu); tjycai@jnu.edu.cn (Jiye Cai)

Abstract

Activation of lymphatic cells is associated with changes in morphology, ultrastructure and adhesion force. We have investigated the activation efficiency of Staphylococcus aureus (SAC) on B-cell chronic lymphatic leukaemia (B-CLL) cells using atomic force microscopy (AFM), and found changes in the above properties. Cell viability and proliferation were measured using Cell Counting Kit-8 (CCK-8) and enzyme-linked immunosorbent assay (ELISA). AFM clearly showed that the volume and nuclear–cytoplasm ratio of cells increased significantly with activated time. It also showed that pseudopodia and immunological synapses began to appear at 24 h. In the activation process, nano-structures of the cell surface became aggregated, and adhesion increased. In conclusion, the results indicate a close relationship between membrane reconstruction and multiplication process of B-CLL cells.

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