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cbin10059-sm-0001-SuppFig-S1.tif2079KFigure S1. Expression of bcl-2 and p53 in atholactone-treated HeLa cells. Cells (1 × 106) were treated with altholactone (0, 20 and 40 µg/mL) for 6 h at 37°C. mRNA of bcl-2 and p53 were RT-PCR amplified and amplicons separated by gel-electrophoresis and visualised by ethidium bromid staining. Glycerol-3-phosphate dehydrogenase (G3PDH) mRNA was used as a housekeeping gene control. Negative control contained only assay buffer.

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