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cbin10081-sm-0001-SupFig-S1.jpg512KFigure S1. Canonical Wnt signalling was not activated by overexpression of TMEM67. HEK 293 cells were transfected with control vector (−) or Flag-tagged-TMEM67 vector (+) for 48. (A) Cells were analysed by Western blotting with antibodies against p-GSK3β (Ser 9) and GSK. β-Actin was visualised as a loading control. (B) Western blots were statistically analysed following the normalisation by using Image J densitometry. The bar graphs represent the level of phosphorylation of GSK3β (n = 3).

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