Downregulation of miRNA-128 sensitises breast cancer cell to chemodrugs by targeting Bax

Authors

  • Shufeng Ji,

    1. Department of General Surgery, Zhujiang Hospital of Nanfang Medical University, Haizhu District of Guangzhou City, Guangdong Province, China
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    • These authors contributed equally to this work.
  • Guoli Shao,

    1. Department of General Surgery, Zhujiang Hospital of Nanfang Medical University, Haizhu District of Guangzhou City, Guangdong Province, China
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    • These authors contributed equally to this work.
  • Xiaobin Lv,

    1. Central Laboratory of Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, No 107 Yanjiang Road, Yuexiu District of Guangzhou, China
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  • Yuan Liu,

    1. Department of General Surgery, Zhujiang Hospital of Nanfang Medical University, Haizhu District of Guangzhou City, Guangdong Province, China
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  • Yingfang Fan,

    1. Oncology Department, Sixth People's Hospital of Shanghai, No 600 Yishan Road, Xuhui District of Shanghai City, China
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  • Aiguo Wu,

    1. Department of General Surgery, Zhujiang Hospital of Nanfang Medical University, Haizhu District of Guangzhou City, Guangdong Province, China
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  • Haiyan Hu

    Corresponding author
    • Oncology Department, Sixth People's Hospital of Shanghai, No 600 Yishan Road, Xuhui District of Shanghai City, China
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Corresponding author: e-mail: xuri1104@164.com

Abstract

miR-128 is more highly expressed in drug-resistant breast cancer samples than in drug-sensitive samples. We have confirmed that Bax is the target of miR-128 by negative post-transcriptional regulation. miR-128 and Bax were detected in the breast cancer cell line, MDA-MB-231, which was then transfected with miR-128 MIMIC (precursor of miR-128) or AMO (antisense-miR-128 oligonucleotides). After transfection, the chemosensitivity of MDA-MB-231 cell was up-regulated with increasing of Bax and inhibition of miR-128.

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