RNAi-mediated MMP-9 silencing inhibits mouse melanoma cell invasion and migration in vitro and in vivo

Authors

  • Zhao-Yong Tang,

    1. College of Biotechnology, Southwest University, Chongqing, China
    2. State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China
    Search for more papers by this author
    • These authors contributed equally to this study.
  • Yang Liu,

    1. College of Biotechnology, Southwest University, Chongqing, China
    Search for more papers by this author
    • These authors contributed equally to this study.
  • Long-Xing Liu,

    1. College of Biotechnology, Southwest University, Chongqing, China
    Search for more papers by this author
  • Xiao-Yan Ding,

    1. State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China
    Search for more papers by this author
  • Hong Zhang,

    1. State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China
    Search for more papers by this author
  • Liao-Qiong Fang

    Corresponding author
    1. State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China
    • College of Biotechnology, Southwest University, Chongqing, China
    Search for more papers by this author

Corresponding author: e-mail: lqfang06@163.com

Abstract

MMP-9 participates in tumour growth, invasion, metastasis and vascularisation. Thus, inhibition of MMP-9 may be involved in the process of tumourigenesis. We have investigated the effect of RNAi-mediated MMP-9 silencing on inhibiting invasion and migration of mouse melanoma cell B16. A specific and optimised siRNA vector was used to target MMP-9 mRNA synthesis in B16 cells. Changes of invasion and migration capability of B16 cell were examined after transfection at different time, and a footpad tumour model was performed to measure the effect of MMP-9 siRNA on melanoma tumourigenesis in vivo. In vitro, down-regulation of MMP-9 expression significantly inhibited B16 cell invasion and migration. In vivo, intratumoural injection of plasmid DNA expressing MMP-9 siRNA every 3 days for three times remarkably inhibited melanoma growth and also suppressed tumour metastasis. The results indicate that RNA-mediated targeting of MMP-9 may have promising applications for the treatment of melanoma.

Ancillary