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FilenameFormatSizeDescription
cbin10115-sm-0001-SupFig-S1.tif1019KFigure S1. Prediction of C/EBPβ binding sites on promoter of FoxO1 gene. The 1.5 kb promoter region of porcine Foxo1 gene contains two putative C/EBPβ core binding motifs (blue).
cbin10115-sm-0002-SupFig-S2.tif845KFigure S2. C/EBPα binds to FoxO1 in vitro. (A and B) C/EBPα interacted with FoxO1 in HEK293T cells. Cells were transiently co-transfected with FoxO1 and C/EBPα with the calcium phosphate method. Forty-eight hours after transfection, cells were lysed, and FoxO1 or C/EBPα proteins are immunoprecipitated with agarose beads and conjugated with anti-FoxO1 or anti-C/EBPα antibodies. The precipitated proteins analysed by Western blot as indicated.
cbin10115-sm-0003-SupFig-S3.tif2010KFigure S3. Knockdown of C/EBPβ inhibits porcine preadipocyte adipogenesis. (A) The Oil red O extraction analysis. (B–E) Quantification of western bands for FoxO1, C/EBPβ, C/EBPα and aP2 by Image J software, respectively. The results are represented as means ± SE of three independent determinations. *P < 0.05, **P < 0.01.
cbin10115-sm-0004-SupFig-S4.tif1734KFigure S4. Knockdown of FoxO1 enhances porcine preadipocyte differentiation and decreased the C/EBPβ expression. (A) The Oil red O extraction analysis. (B and C) Quantification of Western bands for FoxO1 and C/EBPβ by Image J software. (D) Western bands quantified for PPARγ (at Day 0) and FAS (at Days 1, 2 and 6). The results are represented as means ± SE of three independent determinations. *P < 0.05, **P < 0.01.
cbin10115-sm-0005-SupFig-S5.tif1620KFigure S5. Knockdown of both C/EBPβ and FoxO1 enhance preadipocyte differentiation. (A) The Oil red O extraction analysis. (B–E) Quantification of Western bands for FoxO1, C/EBPβ, C/EBPα and aP2 by Image J software, repectively. The results are represented as means ± SE of three independent determinations. *P < 0.05, **P < 0.01.
cbin10115-sm-0006-SupFig-S6.tif1003KFigure S6. Knockdown of both C/EBPβ and FoxO1 enhance preadipocyte differentiation significantly, compared with FoxO1 knockdown alone. (A) The Oil red O extraction analysis. (B and C) Quantification of Western bands for C/EBPα and aP2 by Image J software, respectively. The results are represented as means ± SE of three independent determinations. *P < 0.05, **P < 0.01, compared with scrambled treatment; #P < 0.05, compared with shFoxO1 treatment.

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