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FilenameFormatSizeDescription
cbin10119-sm-0001-SupTab-S1.docx11KTable S1. Details of the human cornea used.
cbin10119-sm-0002-video-S1.mpg17056KVideo S1. Development of polarised outgrowth. A sphere at DIV 64 in a collagen-coated agarose well. The actual duration of imaging was 53 h. Cells first started migrating from the opposite poles of the sphere. The cellular migration to a certain area appears to be inhibited via an unknown mechanism. Scale bar = 100 μm.
cbin10119-sm-0003-video-S2.mpg8052KVideo S2. Non-Brownian motion of a sphere on collagen-coated coverslip. Total duration of this video was 28 h:38 min. During the imaging period, movement of the sphere within the imaging frame was observed in conjunction with cell extension and retraction. Scale bar = 100 μm.
cbin10119-sm-0004-video-S3.mpg20416KVideo S3. En masse migration of a sphere on collagen substrate. A sphere at DIV 64 was seeded in a collagen I-coated agarose well and incubated for 16 h prior to time-lapse imaging. The actual duration of the video presented here is 96 h:35 min. The first 39 h:10 min of video has been removed since there was little movement by the sphere. The boundaries formed by agarose can be seen in the top right corner. This video shows en masse migration of the sphere with clear formation of leading and trailing compartments. The leader cells appeared to undergo classic stages of cellular locomotion; protrusion, adhesion, traction and de-adhesion/tail retraction, while the cells at the rear end continued to retract back into the sphere. Scale bar = 100 μm.
cbin10119-sm-0005-video-S4.mpg16308KVideo S4. Active cellular re-recruitment into a sphere. A sphere at DIV 113 was cultured on collagen-coated coverslip for 6 days prior to imaging. The actual duration of the video was 13 h:29 min. Fibroblastic cells grew out of the sphere from the 8 O'clock position. These cells extended pseudopodia towards a small round cell ∼180 μm away from the sphere and established intercellular contact (indicated by an arrow). The small round cell was then rapidly taken into the sphere. Scale bar = 100 μm.
cbin10119-sm-0006-video-S5.mpg11308KVideo S5. A cell division occurring at the periphery of a sphere. Experimental conditions were the same as in Supplementary Video 4. Imaging took place over 3 h:50 min. Immediately after a cell division seen at the 12 O'clock position from the sphere (indicated by a white arrow), one of the daughter cells retreated back into the sphere while the other migrated away. Scale bar = 100 μm.

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