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Oxidized low-density lipoprotein induces hematopoietic stem cell senescence

Authors

  • Xian-Ping Zhang,

    1. Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, China
    Current affiliation:
    1. Department of Histology and Embryology, Zunyi Medical College, Guizhou, China
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  • Gui-Hai Zhang,

    1. Department of Oncology, Affiliated Hospital of Zunyi Medical College, Guizhou, China
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  • Yu-Ying Wang,

    1. Department of Cell Engineering Key Laboratory, Affiliated Hospital of Zunyi Medical College, Guizhou, China
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  • Jun Liu,

    1. Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, China
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  • Qiang Wei,

    1. Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, China
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  • Chun-Yan Xu,

    1. Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, China
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  • Jian-Wei Wang,

    1. Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, China
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  • Ya-Ping Wang

    Corresponding author
    • Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, China
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Corresponding author: e-mail: ypwangcq@yahoo.cn

Abstract

We have investigated oxidized low-density lipoprotein (ox-LDL) induced senescence in hematopoietic stem cells (HCs). Mouse Sca-1+ HCs were separated and purified using the magnetic activated cell sorting technique. Ox-LDL induced significant senescence in HCs measured by SA-β-Gal staining, and reduced CFU-Mix colony-forming capacity, arresting cells at G0/G1 phase. In agreement with the cell cycle arrest, ox-LDL markedly reduced the expression of CDK4, cyclin D, and cyclin E. As possible contributing factors for cell senescence, ox-LDL also induced cellular oxidative stress and reduced telomerase activity.

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