Methods for rearing Mesostoma ehrenbergii in the laboratory for cell biology experiments, including identification of factors that influence production of different egg types
Article first published online: 26 JUN 2013
© 2013 International Federation for Cell Biology
Cell Biology International
Volume 37, Issue 10, pages 1089–1105, October 2013
How to Cite
Hoang, C., Ferraro-Gideon, J., Gauthier, K. and Forer, A. (2013), Methods for rearing Mesostoma ehrenbergii in the laboratory for cell biology experiments, including identification of factors that influence production of different egg types. Cell Biology International, 37: 1089–1105. doi: 10.1002/cbin.10129
- Issue published online: 5 SEP 2013
- Article first published online: 26 JUN 2013
- Accepted manuscript online: 17 MAY 2013 09:48AM EST
- Manuscript Accepted: 25 APR 2013
- Manuscript Received: 21 JAN 2013
Additional supporting information may be found in the online version of this article at the publisher's website.
Video S1. Video showing the dissection of M. ehrenbergii. The following steps were taken at the following time points: 17:06, M. ehrenbergii is placed into a well made from vacuum grease; 17:07, most of the Ringer's solution is removed from the well to limit movement; 17:08, 10 µL needle is inserted into the testes through the animal's body wall and the testes is sucked out; 17:09, the testes in Ringer's solution mixture is expelled onto a cover slip; 17:10, fibrinogen is added to the mixture and a thin layer of solution is spread over the cover slip; 17:11, thrombin is added over the layer to form the clot.
Video S2. Video showing M. ehrenbergii consuming brine shrimp. At 18:32, brine shrimp was just added and the worm is seen accumulating its food. From 18:40 onwards, a bundle of brine shrimp held together by the glue these animals secrete can be seen around the pharynx of the animal as it consumes its prey.
Video S3. Video showing M. ehrenbergii giving birth to S eggs. There is an initial video sequence displaying the process 30× the actual speed, followed by a second sequence which shows the S eggs being released in real time. The S eggs can be seen released at the following times: 19:01:51, 19:04:07, 19:05:44, and 19:05:45.
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