Insulin facilitates osteoblast differentiation
Article first published online: 23 JUL 2013
© 2013 International Federation for Cell Biology
Cell Biology International
Volume 37, Issue 11, pages 1157–1161, November 2013
How to Cite
Wang, L., Wang, H., Xu, X., Wang, D. and Liu, H. (2013), Insulin facilitates osteoblast differentiation. Cell Biology International, 37: 1157–1161. doi: 10.1002/cbin.10147
- Issue published online: 7 OCT 2013
- Article first published online: 23 JUL 2013
- Accepted manuscript online: 20 JUN 2013 07:04AM EST
- Manuscript Accepted: 27 MAY 2013
- Manuscript Received: 19 APR 2013
- National Natural Science Foundation of China. Grant Number: 30973354
- adipose stem cells;
- genetic engineering;
- insulin-like growth factor 1 receptor;
Tissue engineering of bone has been increasingly used in the bone defect repair. To generate osteoblasts is a major approach, and here we have examined ways of improving the efficiency of producing osteoblasts. Adipose stem cells (ADSC) were prepared from rat mesentery tissue, and transfected with Cbfa1 gene vector or/and IGF-1R gene vector. The cells were stimulated with insulin. Osteocalcin expression by the ADSCs was assessed by quantitative RT-PCR (qRT-PCR), Western blotting and enzyme-linked immunobosorbent assay.
Both genes Cbfa1 and IGF-1R were transfected in ADSCs, as shown by qRT-PCR and Western blotting. Stimulation by insulin in the culture increased osteocalcin expression in ADSCs transfected by both Cbfa1 and IGF-1R, but not in those transfected with only one of these two genes. Osteocalcin in the culture supernatant was also increased by stimulation with insulin.
Thus IGF-1R gene transfer together with insulin stimulation can markedly increase the efficiency of generation of osteoblasts.