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Figure S1. NADPH oxidase (Nox) does not effect adipocyte differentiation. Note that DPI treatment or siRNA transfection of Nox4 did not inhibit adipocyte differentiation. (A and B) Pharmacological Nox inhibitor, DPI, did not attenuate the lipid accumulation (A) and mRNA expression of PPAR-γ and CEBP-β (B). (C and D) Transfection of siRNA for Nox4 also did not attenuate the lipid accumulation (C) and mRNA expression of PPAR-γ and CEBP-β (D). On the contrary, Nox4 silencing slightly induced adipocyte differentiation; **P < 0.01.

Figure S2. Effect of ERK 1/2 inhibition on adipocyte differentiation. Note that ERK1/2 does not mediate adipocyte differentiation. (A and B) Inhibitors of ERK1/2 (U0126) did not reduce lipid accumulation (A) and mRNA expression of PPAR-γ and C/EBP-β (B) in ASCs.

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