Hypoxia induces adipocyte differentiation of adipose-derived stem cells by triggering reactive oxygen species generation
Article first published online: 12 SEP 2013
© 2013 International Federation for Cell Biology
Cell Biology International
Volume 38, Issue 1, pages 32–40, January 2014
How to Cite
Kim, J. H., Kim, S.-H., Song, S. Y., Kim, W.-S., Song, S. U., Yi, T., Jeon, M.-S., Chung, H.-M., Xia, Y. and Sung, J.-H. (2014), Hypoxia induces adipocyte differentiation of adipose-derived stem cells by triggering reactive oxygen species generation. Cell Biology International, 38: 32–40. doi: 10.1002/cbin.10170
- Issue published online: 18 DEC 2013
- Article first published online: 12 SEP 2013
- Accepted manuscript online: 17 AUG 2013 08:09AM EST
- Manuscript Accepted: 29 JUL 2013
- Manuscript Received: 15 APR 2013
- National Research Foundation of Korea. Grant Numbers: 2011-0019636, 2011-0007751, 2012-003803
- NIH. Grant Numbers: HD-034852, AT-004422
Additional Supporting Information may be found in the online version of this article at the publisher's web-site.
Figure S1. NADPH oxidase (Nox) does not effect adipocyte differentiation. Note that DPI treatment or siRNA transfection of Nox4 did not inhibit adipocyte differentiation. (A and B) Pharmacological Nox inhibitor, DPI, did not attenuate the lipid accumulation (A) and mRNA expression of PPAR-γ and CEBP-β (B). (C and D) Transfection of siRNA for Nox4 also did not attenuate the lipid accumulation (C) and mRNA expression of PPAR-γ and CEBP-β (D). On the contrary, Nox4 silencing slightly induced adipocyte differentiation; **P < 0.01.
Figure S2. Effect of ERK 1/2 inhibition on adipocyte differentiation. Note that ERK1/2 does not mediate adipocyte differentiation. (A and B) Inhibitors of ERK1/2 (U0126) did not reduce lipid accumulation (A) and mRNA expression of PPAR-γ and C/EBP-β (B) in ASCs.
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