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Effect of Spilanthes acmella hydroethanolic extract activity on tumour cell actin cytoskeleton

Authors

  • Cristina Pacheco Soares,

    Corresponding author
    1. Universidade do Vale do Paraíba (Univap)/Instituto de Pesquisa e Desenvolvimento (IP&D) – Laboratório de Dinâmica de Compartimentos Celulares, São José dos Campos, SP, Brazil
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  • Valeria Rosseto Lemos,

    1. Centro Universitário Vila Velha Rua Jairo Mattos Pereira, Vila Velha, ES, Brazil
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  • Ary Gomes da Silva,

    1. Centro Universitário Vila Velha Rua Jairo Mattos Pereira, Vila Velha, ES, Brazil
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  • Renan Meyer Campoy,

    1. Universidade do Vale do Paraíba (Univap)/Instituto de Pesquisa e Desenvolvimento (IP&D) – Laboratório de Dinâmica de Compartimentos Celulares, São José dos Campos, SP, Brazil
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  • Carlos Augusto Priante da Silva,

    1. Universidade do Vale do Paraíba (Univap)/Instituto de Pesquisa e Desenvolvimento (IP&D) – Laboratório de Dinâmica de Compartimentos Celulares, São José dos Campos, SP, Brazil
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  • Renato Farina Menegon,

    1. Universidade do Vale do Paraíba (Univap)/Instituto de Pesquisa e Desenvolvimento (IP&D) – Laboratório de Farmacognosia e Química Medicinal, São José dos Campos, SP, Brazil
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  • Iuri Rojahn,

    1. Universidade do Vale do Paraíba (Univap)/Instituto de Pesquisa e Desenvolvimento (IP&D) – Laboratório de Farmacognosia e Química Medicinal, São José dos Campos, SP, Brazil
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  • Walderez Moreira Joaquim

    1. Universidade do Vale do Paraíba (Univap)/Instituto de Pesquisa e Desenvolvimento (IP&D) – Laboratório de Farmacognosia e Química Medicinal, São José dos Campos, SP, Brazil
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Abstract

Numerous natural products have pharmacological activity such that many biologically active compounds have led to the development of cancer chemotherapy drugs. Spilanthes acmella (Asteraceae) is widely cultivated in the State of Pará, Brazil, being employed in folk medicine for its anti-inflammatory, antimicrobial, antioxidant, analgesic, insecticide, and larvicidal properties. However, its cytotoxicity and influence on actin cytoskeleton organisation in tumour cell lines are practically nonexistent. We have verified the cytotoxicity of a hydroethanolic extract of the inflorescence of S. acmella, and examined its effects on the cytoskeleton of tumour cells. Decreasing concentrations of the extract (250, 500 and 1,000 µg/mL) were given to cultures of neoplastic cells (HEp-2). Cytotoxicity was assessed by the MTT test, and the influence on cytoskeleton organisation was examined by fluorescence microscopy. The IC50 of the hydroethanolic extract was 513 µg/mL, confirming the data obtained from the MTT assay that gave high cytotoxicity. The actin cytoskeleton arrangement of HEp2 cells at 500 and 1,000 µg/mL showed depolymerisation of the filaments, causing loss of morphology and consequently compromising cell adhesion.

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