Bone formation in adipose-derived stem cells isolated from elderly patients with osteoporosis: a preliminary study

Authors

  • Ming Jiang,

    1. Department of Stomatology, 107 Hospital of Ji'nan military area, Yantai, Shandong, P.R. China
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    • These authors contributed equally to this work.
  • Xin Wang,

    1. Department of Orthopaedics and Traumatology, Yantai YuHuangDing Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong, P.R. China
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    • These authors contributed equally to this work.
  • Hongzhi Liu,

    1. Department of Orthopaedics and Traumatology, Yantai YuHuangDing Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong, P.R. China
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  • Lugang Zhou,

    1. Department of Orthopaedics and Traumatology, Yantai YuHuangDing Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong, P.R. China
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  • Tingshu Jiang,

    1. Department of Respiratory Medicine, Yantai YuHuangDing Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong, P.R. China
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  • Huihui Zhou,

    1. Department of Pathology, Yantai YuHuangDing Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong, P.R. China
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  • Wei Hao

    Corresponding author
    1. Department of Orthopaedics and Traumatology, Yantai YuHuangDing Hospital Affiliated to Qingdao University Medical College, Yantai, Shandong, P.R. China
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Abstract

We have explored the osteogenic potency of adipose-derived stem cells from osteoporotic patients (opASCs). opASCs were osteogenically induced in vitro with collagen I hydrogel or in culture plate. Detection of alkaline phosphatase (ALPase) and cell mineralisation, and quantitative RT-PCR of collagen I, osteocalcin and bone sialoprotein were undertaken. Proliferation and morphology studies were also performed. After 14 days, opASCs-collagen I hydrogel composite was implanted into nude mice for 4 weeks prior to radiographic and histological analysis. Staining of ALPase and cell mineralisation was strongly positive in opASCs. Fibroblast-like opASCs induced with collagen I hydrogel were evenly distributed and proliferated at a higher rate than in culture plates, showing similar growth curves for both genders. Expression of ALPase activity, cell mineralisation and osteogenic specific genes were higher in opASCs with collagen I hydrogel (male samples had better osteogenicity than female samples) than in culture plates. After implantation for 4 weeks, radiopaque area signifying new bone tissue was observed in opASCs-collagen I hydrogel composite, with no donor gender differences. Thus opASCs with collagen I hydrogel have adequate osteogenic potency and offer new possibilities for osteoporosis-related bone tissue engineering in male and female patients.

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