Present address: 1# Weigang, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Cytoprotection of methionine on hyperthermia-induced damage in bovine mammary epithelial cells
Article first published online: 12 MAY 2014
© 2014 International Federation for Cell Biology
Cell Biology International
Volume 38, Issue 8, pages 971–976, August 2014
How to Cite
Mu, T., Kong, G.-H., Han, Z.-Y. and Li, H.-X. (2014), Cytoprotection of methionine on hyperthermia-induced damage in bovine mammary epithelial cells. Cell Biology International, 38: 971–976. doi: 10.1002/cbin.10271
- Issue published online: 3 JUL 2014
- Article first published online: 12 MAY 2014
- Accepted manuscript online: 7 MAR 2014 01:53AM EST
- Manuscript Accepted: 21 FEB 2014
- Manuscript Received: 11 OCT 2013
- bovine mammary epithelial cells;
- oxidative damage
The protective effects of methionine against hyperthermia-induced damage in bovine mammary epithelial cells (BMEC) were studied. We have investigated the effects of methionine on proliferation, antioxidant activity, and apoptosis of the mammary epithelial cells of dairy cow after heat treatment. The structure of BMEC membrane was damaged by hyperthermia. Methionine (30 and 60 mg/L) efficiently increased cell viability and attenuated morphological damages in hyperthermia-treated BMEC. It significantly reduced lactate dehydrogenase leakage and malondialdehyde formation, whereas superoxide dismutase activity increased significantly. It also increased cell survival and decreased early apoptosis. Methionine therefore is cytoprotective on hyperthermia-induced damage in BMEC by increasing intracellular antioxidant levels and decreasing lipid peroxidation.