Human endometrial stem cells differentiation into functional hepatocyte-like cells

Authors

  • Farzaneh Khademi,

    1. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
    2. Stem Cells Technology Research Center, Tehran, Iran
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  • Masoud Soleimani,

    1. Stem Cells Technology Research Center, Tehran, Iran
    2. Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
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  • Javad Verdi,

    1. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
    2. Department of Applied Cell, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
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  • Seyed Mohammad Tavangar,

    1. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
    2. Department of Pathology, Shariaty Hospital, Tehran University of Medical Sciences, Tehran, Iran
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  • Esmaeil Sadroddiny,

    1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
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  • Mohammad Masumi,

    1. Stem Cells Technology Research Center, Tehran, Iran
    2. Induced Pluripotent Stem Cell Biotechnology Team, Stem Cells Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
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  • Jafar Ai

    Corresponding author
    1. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
    2. Brain and Spinal Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran
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Errata

This article is corrected by:

  1. Errata: Human endometrial stem cells differentiation into functional hepatocyte-like cells Volume 39, Issue 1, 129, Article first published online: 5 January 2015

Abstract

In spite of certain clinical limitations, such as teratoma formation, the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. This study shows human endometrial stem cells (hEnSCs) has exceptional differentiation ability in hepatocyte formation. hEnSCs have high purification rate and immune-tolerance, and can be used as an appropriate substitute for hepatocytes in liver disorders. Differentiation required hepatogenic medium. Quantitative reverse transcription-polymerase chain reaction and immunofluorescent staining of hepatic genes and proteins including cytokeratin 18 (ck18), alpha-fetoprotein (afp), and albumin (alb) were used to assess differentiation. Cells differentiated with a hepatocyte-like morphology and expressed hepatic markers on 30 days of differentiation. The Periodic Acid-Schiff (PAS) reaction showed storage of glycogen, and albumin and afp secretions were also detected. In vitro hEnSCs behave like hepatocyte after differentiation and may be a suitable source of cells in liver regeneration.

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