Nucleolar persistence during spermatogenesis of the genus Rhodnius (Hemiptera, Triatominae)

Authors

  • Kaio Cesar Chaboli Alevi,

    Corresponding author
    1. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista—São José do Rio Preto, São José do Rio Preto, SP, Brazil
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  • Nayara Fernanda da Costa Castro,

    1. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista—São José do Rio Preto, São José do Rio Preto, SP, Brazil
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  • Anna Claudia Campaner Lima,

    1. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista—São José do Rio Preto, São José do Rio Preto, SP, Brazil
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  • Amanda Ravazi,

    1. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista—São José do Rio Preto, São José do Rio Preto, SP, Brazil
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  • Alessandra Morielle-Souza,

    1. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista—São José do Rio Preto, São José do Rio Preto, SP, Brazil
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  • Jader de Oliveira,

    1. Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista—Araraquara, Araraquara, SP, Brazil
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  • João Aristeu da Rosa,

    1. Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista—Araraquara, Araraquara, SP, Brazil
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  • Maria Tercília Vilela de Azeredo Oliveira

    1. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista—São José do Rio Preto, São José do Rio Preto, SP, Brazil
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Abstract

The Triatominae subfamily is comprised of 18 genera and six tribes. The tribe Rhodniini is comprised of two genera (Rhodnius and Psammolestes). Nucleolar persistence is defined by the presence of the nucleolus or nucleolar corpuscles during the meiotic metaphase. To date, this phenomenon has been described for 13 species of triatomine that are included in the genera Triatoma, Rhodnius, and Panstrongylus. Thus, because the phenomenon of nucleolar persistence has been described in only two species of the genus Rhodnius, we have analyzed the nucleolar behavior during spermatogenesis of eight species of the genus Rhodnius (R. colombiensis, R. montenegrensis, R. nasutus, R. neglectus, R. neivai, R. pictipes, R. prolixus, and R. robustus), with a focus on nucleolar persistence. By means of cytogenetic analysis with silver ions, nucleolar behavior during spermatogenesis is described in the eight species of Rhodnius analyzed. In all of them nucleolar behavior was similar and the phenomenon of nucleolar persistence was often observed. Therefore, we confirm nucleolar persistence as a peculiarity of the genus Rhodnius. However, it is emphasized that new cytogenetic analysis should be performed in the Triatominae subfamily, more specifically among the 15 genera that do not exhibit the nucleolar behavior described, in order to assess whether this phenomenon is truly a synapomorphy of these hematophagous insects.

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