We have assessed the effect of bradykinin and histamine on the cytosolic free calcium concentration ([Ca2+]i) of bovine adrenal medulla capillary endothelial cells (BAMCECs). To measure [Ca2+]i changes in BAMCECs the intracellular fluorescent probe, fluo-3 AM, was used. Bradykinin (3 µM) produced a transient monophasic increase in [Ca2+]i, which was depressed by B1650 (0.1 µM), a B2-bradykinin receptor antagonist (D-Arg-[Hyp3, Thi5,8, D-Phe7]-Bradykinin). Similarly, increase in [Ca2+]i induced by histamine was also depressed by tripolidine (0.1 µM), an H1-histamine receptor antagonist. [Ca2+]i increase induced by both agonists was unaffected in the absence of extracellular Ca2+ or presence of antagonists of voltage operated Ca2+ channels (VOCCs). Thapsigargin (1 µM) did not abolish the increase of [Ca2+]i produced by bradykinin, but abolished that of histamine. In contrast, caffeine (100 µM), abolished the [Ca2+]i response induced by bradykinin (3 µM), but did not affect the [Ca2+]i increase induced by histamine (100 µM). The results indicate the presence of B2 bradykinin- and H1 histamine-receptors in BAMCECs. Liberation of Ca2+ induced by both agonists occurs through 2 different intracellular mechanisms. While bradykinin activates a sarco(endo) plasmic reticulum (SER) containing a SER Ca2+-ATPase (SERCA) thapsigargin-insensitive, histamine activates a SER containing a SERCA thapsigargin-sensitive. We suggest that the increase in [Ca2+]i induced by bradykinin and histamine could be of physiological relevance, modulating adrenal gland microcirculation.