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Entrapping Flavin-Containing Monooxygenase on Corrugated Silica Nanospheres and their Recyclable Biocatalytic Activities

Authors

  • Archana A. Biradar,

    1. Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854 (USA), Fax: (+1) 732-445-2970 http://rutchem.rutgers.edu/?q=node/565
    2. Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, New Jersey 08854 (USA), Fax: (+1) 732-445-2970
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  • Ankush V. Biradar Dr.,

    1. Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854 (USA), Fax: (+1) 732-445-2970 http://rutchem.rutgers.edu/?q=node/565
    2. Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, New Jersey 08854 (USA), Fax: (+1) 732-445-2970
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  • Tewodros Asefa Prof.

    1. Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854 (USA), Fax: (+1) 732-445-2970 http://rutchem.rutgers.edu/?q=node/565
    2. Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, New Jersey 08854 (USA), Fax: (+1) 732-445-2970
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Abstract

Synthetic methods and biocatalytic activities of new classes of heterogeneous biocatalysts by immobilizing flavin-containing monooxygenase on corrugated and nanoporous silica nanospheres are reported. The nanoporous and corrugated silica nanospheres are synthesized by etching silica nanospheres with aqueous KOH solution. The etched nanospheres are proven to have increased surface area, corrugated, cage-like external surfaces, and, most importantly, more accessible and well-suited surfaces to immobilize bigger molecules, such as enzymes. Furthermore, the etched silica nanospheres contain hydrophilic and silanol groups that are conducive for anchoring enzymes. By utilizing the structures of the etched silica nanospheres, effective immobilization of flavin-containing monooxygenase 1 (FMO1) is demonstrated. The FMO1 immobilized etched silica nanospheres have shown efficient and recyclable biocatalytic activity for nicotine oxidation.

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