Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non-Covalent Attachment to Anionic Supports using Zbasic2 Module

Authors

  • Johanna Wiesbauer,

    1. Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz (Austria), Fax: (+43) 316-873-8434
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  • Dr. Juan M. Bolivar,

    1. Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz (Austria), Fax: (+43) 316-873-8434
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  • Dr. Mario Mueller,

    1. Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz (Austria), Fax: (+43) 316-873-8434
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  • Margaretha Schiller,

    1. Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz (Austria), Fax: (+43) 316-873-8434
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  • Prof. Dr. Bernd Nidetzky

    Corresponding author
    1. Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz (Austria), Fax: (+43) 316-873-8434
    2. Co-affiliation of B. Nidetzky: Austrian Research Center of Industrial Biotechnology (ACIB) GmbH, Petergasse 14, 8010 Graz (Austria)
    • Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz (Austria), Fax: (+43) 316-873-8434

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Abstract

original image

Charge is the message: Non-covalent attachment to anionic supports (red) through a positively charged Zbasic2 (blue) module is a powerful approach for reversible oriented immobilization of enzymes (pI 4-7) on ion-exchange resins commonly used in the protein and enzyme technology. High protein loading and near-native catalytic effectiveness of the carrier-bound enzyme can be achieved.

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