Get access

A Novel Synthesis of Bioactive Catechols by Layer-by-Layer Immobilized Tyrosinase in an Organic Solvent Medium

Authors

  • Dr. Melissa Guazzaroni,

    1. Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo (Italy), Fax: (+39) 0761-357242
    Search for more papers by this author
  • Dr. Marina Pasqualini,

    1. Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo (Italy), Fax: (+39) 0761-357242
    Search for more papers by this author
  • Dr. Giorgia Botta,

    1. Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo (Italy), Fax: (+39) 0761-357242
    Search for more papers by this author
  • Prof. Raffaele Saladino

    Corresponding author
    1. Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo (Italy), Fax: (+39) 0761-357242
    • Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo (Italy), Fax: (+39) 0761-357242
    Search for more papers by this author

Abstract

Commercial and extracted mushroom tyrosinases were supported on Eupergit C 250 L and protected by a coating of oppositely charged polyelectrolytes by means of the layer-by-layer technique. The kinetic parameters (Km, Vmax, and Vmax/Km) of these novel biocatalysts in both organic and aqueous media were evaluated, showing tyrosinase to be more reactive in organic solvent than in buffer solution. Heterogeneous tyrosinase systems were used for the oxidation of a large group of phenol derivatives to the corresponding catechols. Different enzyme reactivities were found depending on the substrate structure. The catalyst activity was retained for successive runs. Catechols are difficult to synthesize through traditional chemical methods under environmentally friendly conditions; the use of immobilized tyrosinase opens a novel synthetic alternative to this interesting biologically active family of substances.

Get access to the full text of this article

Ancillary