Get access
Advertisement

Increasing the Productivity of Peroxidase-Catalyzed Oxyfunctionalization: A Case Study on the Potential of Two-Liquid-Phase Systems

Authors

  • Ekaterina Churakova,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft (The Netherlands), Fax: (+31) 015-2781415
    Search for more papers by this author
  • Prof. Dr. Isabel W. C. E. Arends,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft (The Netherlands), Fax: (+31) 015-2781415
    Search for more papers by this author
  • Dr. Frank Hollmann

    Corresponding author
    1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft (The Netherlands), Fax: (+31) 015-2781415
    • Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft (The Netherlands), Fax: (+31) 015-2781415
    Search for more papers by this author

Abstract

Biocatalysis, which occurs mostly in aqueous reaction media, is generally incompatible with the hydrophobic nature of many reactants. This may be overcome by using the two-liquid-phase system (2 LPS) approach. We demonstrate the beneficial application of the 2 LPS approach for the photobiocatalytic oxyfunctionalization of thioanisole. Compared to aqueous reaction conditions, the 2 LPS approach not only allowed for higher substrate loadings (without the use of additional solvents) but also increased the productivity and robustness of the overall reaction.

Get access to the full text of this article

Ancillary