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Dye Degradation by Layer-by-Layer Immobilised Peroxidase/Redox Mediator Systems

Authors

  • Prof. Dr. Raffaele Saladino,

    Corresponding author
    1. Department of Agrobiology and Agrochemistry, University of Tuscia via S. Camillo de Lellis, I-01100 Viterbo (Italy), Fax: (+39) 0761-357242
    • Department of Agrobiology and Agrochemistry, University of Tuscia via S. Camillo de Lellis, I-01100 Viterbo (Italy), Fax: (+39) 0761-357242
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  • Dr. Melissa Guazzaroni,

    1. Department of Agrobiology and Agrochemistry, University of Tuscia via S. Camillo de Lellis, I-01100 Viterbo (Italy), Fax: (+39) 0761-357242
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  • Dr. Claudia Crestini,

    1. Department of Chemical Science and Technology, University of Rome Tor Vergata via della Ricerca Scientifica 1, I-00133 Rome (Italy)
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  • Dr. Marcello Crucianelli

    Corresponding author
    1. Department of Physical and Chemical Sciences, University of L'Aquila via Vetoio, I-67100 Coppito (AQ) (Italy), Fax: (+39) 0862-433753
    • Department of Physical and Chemical Sciences, University of L'Aquila via Vetoio, I-67100 Coppito (AQ) (Italy), Fax: (+39) 0862-433753
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Abstract

Horseradish peroxidase (HRP) was immobilised on Eupergit C 250 L resin coated with poly-electrolytes, or by entrapment inside pre-formed layer-by-layer (LbL) micro-capsules of poly-electrolytes. In these systems, namely HRP/E-LbL, HRPm/LbL and HRPm/LbLp, the native enzyme retained its catalytic activity. Immobilised HRP showed a significant activity in the oxidation of selected azo, quinoline and fluorone dyes with H2O2 as the primary oxidant under mild experimental conditions, and HRPm/LbL was the best catalyst. A comparison between the catalytic efficiency of different redox mediators for HRP activity was made by using 1-hydroxybenzotriazole (HOBt), violuric acid (VLA) and veratrylic alcohol (VA). As a general trend, azo dyes were degraded in higher yields, and HOBt was the best mediator for the oxidation. The degradation yield increased on increasing the reaction time and reached the highest value after 12 h, which is comparable with that observed for native HRP. Notably, HRPm/LbL retained its catalytic activity for more runs.

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