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Keywords:

  • enantioselectivity;
  • enzymes;
  • mutagenesis;
  • proteins;
  • structure elucidation
Thumbnail image of graphical abstract

Game bOYE: The stereoselectivity and quaternary structure of enoate reductase, of the OYE family, has previously been predicted directly from its primary structure. In this work, this hypothesis is tested by using a mutagenesis approach and by evaluating a new natural enoate reductase.