Hydroxynitrile Lyases Do Not Catalyse the Promiscuous Addition of Cyanide to Imines

Authors

  • Dr. Guzman Torrelo,

    1. Gebouw voor Scheikunde, Biokatalysis & Organische Chemie, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft (Netherlands), Fax: (+31) 15-278-1415
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  • Dr. Jianfeng Jin,

    1. Gebouw voor Scheikunde, Biokatalysis & Organische Chemie, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft (Netherlands), Fax: (+31) 15-278-1415
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  • Prof. Ulf Hanefeld

    Corresponding author
    1. Gebouw voor Scheikunde, Biokatalysis & Organische Chemie, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft (Netherlands), Fax: (+31) 15-278-1415
    • Gebouw voor Scheikunde, Biokatalysis & Organische Chemie, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, 2628 BL Delft (Netherlands), Fax: (+31) 15-278-1415
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Abstract

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Blank reaction: Ten years ago, it was reported that hydroxynitrile lyase (HNL) from defatted almond meal shows catalytic promiscuity in the addition of cyanide to imines. Here we provide experimental evidence that this addition occurs in a non-enzymatic reaction, and two structurally different HNLs do not catalyse this reaction.

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