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Keywords:

  • catalytic promiscuity;
  • molecular modeling;
  • enzymes;
  • molecular dynamics;
  • nucleophilic addition
Thumbnail image of graphical abstract

Blank reaction: Ten years ago, it was reported that hydroxynitrile lyase (HNL) from defatted almond meal shows catalytic promiscuity in the addition of cyanide to imines. Here we provide experimental evidence that this addition occurs in a non-enzymatic reaction, and two structurally different HNLs do not catalyse this reaction.