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Fluorescence-Based Assay for the Optimization of the Activity of Artificial Transfer Hydrogenase within a Biocompatible Compartment

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Abstract

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The time capsules: The transfer hydrogenation of an enone-bound fluorogenic compound by an artificial metalloenzyme leads to the release of fluorescent compound umbelliferone. Upon encapsulation of the hybrid catalyst inside a biocompatible compartment, the activity of the transfer hydrogenase is maintained for several months, even at micromolar concentrations.

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