These authors contributed equally to this work.
Enantioselective Synthesis of N-Substituted Aspartic Acids Using an Engineered Variant of Methylaspartate Ammonia Lyase
Article first published online: 7 MAR 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 5, Issue 6, pages 1325–1327, June 2013
How to Cite
Puthan Veetil, V., Raj, H., de Villiers, M., Tepper, P. G., Dekker, F. J., Quax, W. J. and Poelarends, G. J. (2013), Enantioselective Synthesis of N-Substituted Aspartic Acids Using an Engineered Variant of Methylaspartate Ammonia Lyase. ChemCatChem, 5: 1325–1327. doi: 10.1002/cctc.201200906
- Issue published online: 30 MAY 2013
- Article first published online: 7 MAR 2013
- Manuscript Received: 6 DEC 2012
- Division of Chemical Sciences of the Netherlands Organisation of Scientific Research. Grant Numbers: 700.54.401, 700.59.042
- amino acids;
- Michael addition
Unnatural reactions: We report the synthesis of a large variety of N-substituted aspartic acids by addition of structurally diverse amines to fumaric acid; the reactions are catalyzed by a previously engineered variant of methylaspartate ammonia lyase. The additions are highly enantioselective and yield only the L enantiomers of the corresponding amino acid products (>99 % ee).