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Activity, Recyclability, and Stability of Lipases Immobilized on Oil-Filled Spherical Silica Nanoparticles with Different Silica Shell Structures

Authors

  • Dr. Yasutaka Kuwahara,

    1. Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7457
    2. Present address: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569 (Japan)
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  • Takato Yamanishi,

    1. Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7457
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  • Dr. Takashi Kamegawa,

    1. Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7457
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  • Dr. Kohsuke Mori,

    1. Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7457
    2. Unit of Elements Strategy Initiative for Catalysts & Batteries, Kyoto University, Katsura, Kyoto 615-8510 (Japan)
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  • Prof. Dr. Hiromi Yamashita

    Corresponding author
    1. Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7457
    2. Unit of Elements Strategy Initiative for Catalysts & Batteries, Kyoto University, Katsura, Kyoto 615-8510 (Japan)
    • Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7457
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Abstract

Candida antarctica lipase A was immobilized on spherical silica nanoparticles with oil-filled core and oil-induced mesoporous silica shell with different silica shell structures. The immobilization of enzymes was achieved by directly adding enzymes to the oil-in-water emulsion system under ambient synthesis conditions, and the silica shell structure was controlled by the addition of the cosolvent ethanol to the initial synthesis medium. Detailed structural analysis revealed the formation of oil-filled spherical silica nanoparticles with 3.4–4.2 nm mesopores randomly arranged in the silica shell; the thickness and pore characteristics of these pores markedly changed with the addition of ethanol. The retention of the enzyme activity during biocatalysis was significantly affected by the structural properties of the silica shells, and it was found that a thick and dense silica shell is essential to afford an active, recyclable, and stable biocatalyst. Furthermore, the oil encapsulated within the core cavity was found to play an important role in achieving a high catalytic efficiency.

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