These authors contributed equally to this work.
Expanding the Scope of Laccase-Mediator Systems
Article first published online: 3 JUL 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 5, Issue 10, pages 3027–3032, October 2013
How to Cite
Könst, P., Kara, S., Kochius, S., Holtmann, D., Arends, I. W. C. E., Ludwig, R. and Hollmann, F. (2013), Expanding the Scope of Laccase-Mediator Systems. ChemCatChem, 5: 3027–3032. doi: 10.1002/cctc.201300205
- Issue published online: 1 OCT 2013
- Article first published online: 3 JUL 2013
- Manuscript Revised: 28 MAY 2013
- Manuscript Received: 19 MAR 2013
- Deutsche Bundesstiftung Umwelt
- ChemBioTec. Grant Number: AZ13253
- enzyme catalysis;
The laccase-mediator system (LMS) for the regeneration of oxidised nicotinamide co-factors was revisited to broaden the mediator scope. Among the 18 mediators screened, acetosyringone, syringaldehyde and caffeic acid excelled with respect to activity and stability under process conditions. The LMS based on the laccase from Myceliophthora thermophila and acetosyringone was further investigated and applied to promote the nicotinamide adenine dinucleotide (NAD+)-dependent oxidation of glucose as well as the oxidative lactonisation of 1,4-butanediol to the corresponding γ-butyrolactone.